So there was a cell study published recently that took human and rat lung cells and exposed them to both cigarette smoke and e-cig vapor (with and without nicotine). Dr. Farsilinos responded saying that the study was bogus based on a detail he saw in one of the charts.
What happened is they were measuring the nicotine in molarity and not in milliliters. For those of you unfamiliar, 1M = 1 molar of solute in 1 liter of a solution. So, essentially 1 molar (1M) = 1 mol/L.
Dr F. concluded that they were using concentrations of nicotine that would never been seen in real life conditions (see the link above where he explains).
Dr. F's detraction resulted from a chart that listed the following concentrations of nicotine they applied to the cells. They we
.1 mM
.5 mM
2.5 mM
5 mM
10 mM
(A mM = millimole or 1/1000th of a mole).
Now, the molar mass of nicotine is 162.23g/mol. This means that 1M of nicotine = 162.23g. Since 1M = 1mol/liter, this means that there would be 162.23g of nicotine in one liter of their solution.
Now here's where I am wondering if Dr. F messed up his calculation. A millimole = .001 M. Thus a millimole of nicotine = .16223g (let's round to .16g). That would be .16g/L.
Now .1 mM of nicotine = 160g x 0.0001 = .016g/L. And so on for the rest of the chart presented in the paper. Here would be the conversions in grams per liter:
.5 = .08g/L
2.5 = .4g/L
5 = .8g/L
10 = 1.6g/L
Now, you are probably thinking, "Yes, but you have grams per liter. We need milligrams per milliliter. Therefore, you would need to move the decimal three places to the right. Hence, .016g/L would equal 16mg/mL. Right?"
Wrong. 0.016g/L = 0.016mg/mL. It's the same. It's something we learn in elementary school called cancellation.
The density of pure nicotine is 1.01g/cm^3. This equates 1:1 with mg/mL. Thus, the density of pure nicotine (undiluted) would be 1.01g/mL or 1010mg/mL.
Dr. F, is claiming that 10 mM =1600mg/mL. This is impossible as that is more nicotine than is possible to fit into one mL, even if it was pure and undiluted. (As I said above, pure nicotine is 1010mg/mL).
I conclude that Dr. F. is wrong when he says they were using 16mg/mL, 80mg/mL, 400mg/mL, 800mg/mL, and especially 1600mg/mL (impossible), etc. He moved the decimal place over when he shouldn't have. Or perhaps I am completely wrong and talking out my behind (I am not a chemist and admit I could be totally confused about molarity and the densities and masses of the chemicals here).
I know we have chemists here. I would like it if an expert would show me the error of my ways, which I fully admit is possible. Again, I am no expert. I'm just a guy who thought that surely these researchers cannot be so silly as to use outrageous amounts of nicotine such as 1600mg/mL!
First published study showing that Juul can deliver a higher and faster boost in blood nicotine than most other e-cigarettes and similar to a cigarette. Nicotine Absorption Profile Among Pod-Based Electronic Nicotine Delivery System Users Any thoughts?
Hi,
Unfortunately they are about to ban nicotine E juice in my country in 6 months time. I was stocking up on my regular E juice but this is not really a long term feasible solution.
Luckily I vape unflavoured, so I figure it shouldn't be too hard to make myself.
I had a bit of a look around and found liquid nicotine, up to 250mg/ml, or nicotine salts, both USP, whatever that means.
Just wondering what the best nicotine product would be for long term storage and quality. If someone could recommend a vendor and product that would be great. I normally vape 70/30 pg juice from Heather's heavenly vapes.
I would prefer a liquid than a salt unless there is a big difference in shelf life.
Thanks for your advice
I just came across this article, claiming that even nicotine-free e-liquids contain lung-harming substances like acrolein:
[URL='http://lungcancernewstoday.com/2015/06/08/nicotine-free-e-cigarette-vapor-also-damages-lung-cells/']Nicotine-Free E-Cigarette Vapor Also Damages Lung Cells Lung Cancer News Today[/URL]
What I hate about articles such as these is that they're almost always posted with some scary headline and usually end up saying there isn't a proper research into topic or they're awaiting further investigations.
Anyhow, can anyone comment on what 'acrolein' is? I've heard of formaldehyde being created when vaping on high wattage, but this is the first time I hear of acrolein.
I was in a bar / restaurant a couple of days ago and noticed another person vaping (guy looked to be in his late 60s). The guy was using what looked like a non-adjustable EGO style stick battery with what looked like an 'old-school' Kanger T3 clearomizer on top.
I struck up a conversation with him for just a moment and asked him what type of juice he was vaping ... The guy never told me the brand or flavor, but said it was 0mg Nic when I asked him.
That was basically the end of our conversation.
My question to you folks is this .... Why would anyone keep vaping if they had no nicotine in there juice? For me, the reason I vape in the first place is to keep off cigarettes. I am obviously addicted to nicotine, and have replaced the nicotine from cigarettes for the nicotine found in my juice.
If I could wean myself completely off nicotine, I think at that point I would just give up on vaping altogether. Someday I would like to be nicotine free, but for now, I just can't see it in my future.
BTW .... I have not smoked a cigarette for 15 months, but I am still vaping on my fairly strong 16mg Nic DIY juice.
...if you do nothing.
Federal Flavor Ban & Vape Taxation
Goes Up for Vote This
Thursday 2/27/2020 in the House.
HR 2339
Go here to notify your Congress people and the President (easy peasy)...
Vaping Prohibition & Taxation is On the Table in Congress
From CASAA...
••••••••••••••••••••••••••••••••••••••••••••••••••••••••••••••••••
"This [Federal Flavor Ban] legislation is being supported by an army of Bloomberg-funded anti-harm reduction groups to the tune of $160,000,000.
At the same time, it is likely that a tax bill, HR 4742, which is attempting to enact tax parity between vapor products and combustible cigarettes, will also be taken up by the full House."
••••••••••••••••••••••••••••••••••••••••••••••••••••••••••••••••••
Text - H.R.4742 - 116th Congress (2019-2020): To amend the Internal Revenue Code of 1986 to impose a tax on nicotine used in vaping, etc.
‘‘“(h) Nicotine.—On taxable nicotine, manufactured in or imported into the United States, there shall be imposed a tax equal to the dollar amount specified in section 5701(b)(1) (or, if greater, $50.33) per 1,810 milligrams of nicotine (and a proportionate tax at the like rate on any fractional part thereof).”
Click to expand...
You wanna pay $2,780.00 extra (plus any state taxes)
for 1 liter of 100mg/ml nic base?
You wanna be forced to vape "tobacco" flavors only?
By all means, do nothing.
I was just watching MSNBC and they said (paraphrased) that a study from Belgium has noted that people who smoke seem to be much less likely to get COVID, and they believe it may be because both nicotine and COVID bind to the same cell receptors (or some thing like that - I'm not a medical person lol), so they think nicotine might block COVID from being able to attach to the cell. They're looking into the connection, but say it's waaayyyy too soon to know anything for sure yet. Meanwhile, in the interim, as I refill my vape...
Here's a link to an article (there were actually several articles on google about this): Smokers 'four times less likely' to contract Covid-19, prompting nicotine patch trials on patients
Okay, so I Vape in very “precise” ways but I cannot figure out how to calculate (do the proper math) on how much I nicotine I am consuming. Can someone help? I have been vaping the exact same way daily for a long time, but still don’t know how much nicotine I get (just an estimate) per session because I suck at math...
Details:
1.) I vape 3mg (3mg of nicotine, per ml of juice) of nicotine using a “Smok Nfix” that holds a 3ml pod of nicotine juice. So that’s 9mg of nicotine, per pod.
2.) I set the wattage to exactly 22W on the vape, always, and it is a MTL pod device.
3.) I take EXACTLY 5 puffs over a period of 5-10 mins. Every puff is EXACTLY 5 seconds. Sometimes only 4 seconds, but just say 5 to make it easier. I use my watch or phone’s timer to make certain the timing is exact. I inhale the same way every time. So 5 puffs X 5 seconds, per puff, equals 25 seconds total for each vape session. After those 5 puffs, I don’t vape again (at all) for 1-3 hours, just depending.
....But how do I figure out how much nicotine I am getting in those 5 puffs? I know that in my Nfix pod there is 9mg of nicotine, in each pod.
I also believe 1 full pod (3ML) gives approximately 325 seconds worth of puffs, with each puff being only 1 SECOND. I only tested this one time so I am not certain, but it’s an estimate.
Can anyone help me do the right math here?
I also still smoke ultra light Marlboro cigarettes (from what I read on multiple sources you only absorb about 0.5mg to 0.7mg of nicotine per cig on the ultra lights) but only 1-3 cigarettes per day.
But I am wondering if my 5 puffs on the vape (5 puffs @ 5 second each) is even remotely close to being equal to an ultra light cigarette that has about 0.5mg nicotine per cig?
Thanks, I suck at math so if anyone can help lol
If nicotine did not exist in any form of ecig product, would the government be looking to tax it all?
When I do not have nicotine in my body I become restless, irritable and generally unglued physically and emotionally until I put nicotine in my body.
I use 3mg nic but vape at least 21mg of nic per day....at least and probably more.
Many say nicotine by itself is not addictive. Through 40 plus years of using it I strongly disagree.
The vapors cause has a double edge to it. The government will eventually tax all ecig products but nicotine is the very reason the government can justify coming into the vape shops and tax to begin with
Hello! So, for whatever reason, pre-employment nicotine testing is becoming a thing in my field. I use 6 mg juice, so I'm just going to switch to 0 mg. Everything I've read says 2 weeks should be good to pass the test. My question is, how much residual nicotine will be left in the vape from previously using 6 mg? I took it apart and cleaned it.... Is that sufficient? Or am I still consuming enough nicotine to fail a test?
Thanks!!
So I've been mouth to lung vaping since I started vaping. Started at 24 mg with the Halo Triton and when I upgraded to the iStick and the Nautilus I lowered my nicotine down to 15 mg. It's been fine.......getting enough nicotine so I don't crave analogs, not getting too much nicotine so that I start feeling sick. Throat hit has been fine and not uncomfortable.
Yesterday I started doing lung hits with the iStick and the Nautilus and with the greater amount of vapor I can produce, I stepped the nicotine down to 12 mg since I also started to get a little lightheaded (I suppose with the greater amount of vapor I am also taking in more nicotine, even though nicotine absorption through the lungs is not supposed to be as good as through the mouth and nose). The juice that I am using is also a 70VG/30PG mix as compared to the 50/50 I was vaping before. However this morning I woke up with an irritated throat and the vape (even when switching back to mouth to lung) feels more harsh.
Do I need to step down the nicotine level even more to 6 mg? Is this normal when going from mouth to lung to direct lung hits? I'm not subohming, so I didn't expect that I would need to drop it down that much. I'm afraid that if I drop it down too much, then I'm going to have more cravings and will need to vape constantly.